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Wiley InterScience | |||||||
  Plant BreedingVolume 126 Issue 4, Pages 410 - 415 Published Online: 25 Apr 2007 © 2010 Blackwell Verlag GmbH
Abstract | References | Full Text: HTML, PDF (Size: 648K) | Related Articles | Citation Tracking  Automation of DNA marker analysis for molecular breeding in crops: practical experience of a plant breeding company Copyright 2007 Svalöf Weibulll AB Journal compilation 2007 Blackwell Verlag, Berlin KEYWORDS DNA marker analysis • marker-assisted selection • genome characterization • automation • practical plant breeding • PCR ABSTRACTIn modern plant breeding, DNA marker analyses are of increasing importance and, as the methods become more widely adopted, the capacity for high-throughput analyses at low cost is crucial for its practical use. Automation of the analysis processes is a way to meet these requirements. In order to achieve this, while keeping adequate flexibility in the analysis processes, Svalöf Weibull AB (SW) has developed a fully automated polymerase chain reaction system. It has been evaluated on barley and canola lines and is capable of analysing up to 2200 samples per day at a cost of 0,24 € per analysis for marker-assisted selection and quality control of genetically modified organisms. A detailed description of this system is given, and improvements to the throughput and applications are discussed. Received March 10, 2006/Accepted June 17, 2006 Communicated by F. Ordon | 
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