Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis

doi:10.1111/j.1365-313X.2007.03103.x

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Systems Maintenance, Monday, 15th February 2010

Wiley InterScience

The Plant Journal

Volume 50 Issue 6, Pages 950 - 957

Published Online: 7 Jun 2007

Published in association with the Society for Experimental Biology

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Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis

Shivakumar P. Devaiah ^1,2 , Xiangqing Pan ^1,2 , Yueyun Hong ^1,2 , Mary Roth ³ , Ruth Welti ³ and Xuemin Wang ^1,2,*

¹ Department of Biology, University of Missouri, St Louis, MO 63121, USA ,
² Donald Danforth Plant Science Center, St Louis, MO 63132, USA , and
³ Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506, USA

Correspondence to *(fax +1 314 516 6233; e-mail wangxue@umsl.edu).

KEYWORDS

seed deterioration • germination • seed oil content • polyunsaturated fatty acids • phospholipase D • lipid oxidation

ABSTRACT

Seed aging decreases the quality of seed and grain and results in agricultural and economic losses. Alterations that impair cellular structures and metabolism are implicated in seed deterioration, but the molecular and biochemical bases for seed aging are not well understood. Ablation of the gene for a membrane lipid-hydrolyzing phospholipase D (PLDα1) in Arabidopsis enhanced seed germination and oil stability after storage or exposure of seeds to adverse conditions. The PLDα1-deficient seeds exhibited a smaller loss of unsaturated fatty acids and lower accumulation of lipid peroxides than did wild-type seeds. However, PLDα1-knockdown seeds were more tolerant of aging than were PLDα1-knockout seeds. The results demonstrate the PLDα1 plays an important role in seed deterioration and aging in Arabidopsis. A high level of PLDα1 is detrimental to seed quality, and attenuation of PLDα1 expression has the potential to improve oil stability, seed quality and seed longevity.

Received 10 October 2006; revised 29 January 2007; accepted: 6 February 2007.

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1365-313X.2007.03103.x About DOI