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Wiley InterScience | ||||||||
![]() Journal of Medical PrimatologyVolume 36 Issue 1, Pages 25 - 32 Published Online: 29 Sep 2006 © 2010 John Wiley & Sons A/S
Abstract | References | Full Text: HTML, PDF (Size: 513K) | Related Articles | Citation Tracking ORIGINAL ARTICLE Polymerase chain reaction detection of Clostridium perfringens in feces from captive and wild chimpanzees, Pan troglodytes Copyright 2007 The Authors Journal compilation 2007 Blackwell Munksgaard KEYWORDS alpha toxin • conservation • health monitoring • nested PCR • non-invasive •
Pan troglodytes schweinfurthii
•
Pan troglodytes verus
Abstract
Background For veterinary management of non-human primates in captivity, and conservation of wild-living primates, management of their health risks is necessary. Incidences of pathogenic bacteria in the fecal specimens are considered as one of the useful indicators for non-invasive health monitoring. Methods We carried out the detection of Clostridium perfringens in feces from captive and wild chimpanzees by the rapid polymerase chain reaction method. Results The bacterium was detected in most fecal specimens (80%) in captive chimpanzees. Contrarily, the detection rate in the wild chimpanzees was low, with 23% (n = 12) of 53 fecal samples from the Bossou group, Guinea, and 1.2% (1/81) in the Mahale group, Tanzania. Conclusions These results show that the intestinal microflora differs between Pan populations under various living conditions, being influenced by their diet and environment. Accepted May 10, 2006. |