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Wiley InterScience | |||
  Journal of Synchrotron RadiationVolume 14 Issue 1, Pages 92 - 98 International Union of Crystallography, 2010 Distributed on behalf of the International Union of Crystallography
Abstract | Full Text: HTML, PDF (Size: 547K) | Related Articles | Citation Tracking  Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry Copyright International Union of Crystallography, 2007 KEYWORDS single-crystal microspectrophotometry • reaction intermediates • structural enzymology • tryptophan tryptophylquinone • methylamine dehydrogenase. ABSTRACTX-ray exposure during crystallographic data collection can result in unintended redox changes in proteins containing functionally important redox centers. In order to directly monitor X-ray-derived redox changes in trapped oxidative half-reaction intermediates of Paracoccus denitrificans methylamine dehydrogenase, a commercially available single-crystal UV/Vis microspectrophotometer was installed on-line at the BioCARS beamline 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox state of the intermediates during X-ray exposure permitted the creation of a general multi-crystal data collection strategy to generate true structures of each redox intermediate. Received 26 May 2006, accepted 27 November 2006 | 
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