Identification of 10 882 porcine microsatellite sequences and virtual mapping of 4528 of these sequences

doi:10.1111/j.1365-2052.2007.01609.x

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Wiley InterScience

Animal Genetics

Volume 38 Issue 4, Pages 401 - 405

Published Online: 9 Jun 2007

Published on behalf of the International Society for Animal Genetics

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SHORT COMMUNICATION

Identification of 10 882 porcine microsatellite sequences and virtual mapping of 4528 of these sequences

P. Karlskov-Mortensen*, Z. L. Hu ^† , J. Gorodkin*, J. M. Reecy ^† and M. Fredholm*

*Department of Animal and Veterinary Basic Sciences, Division of Genetics, Faculty of Life Sciences, University of Copenhagen, Denmark . ^† Department of Animal Science, Center for Integrated Animal Genomics, Iowa State University, 2255 Kildee Hall, Ames, IA 50011-3150, USA

Correspondence to P. Karlskov-Mortensen, Department of Animal and Veterinary Basic Sciences, Division of Genetics, Faculty of Life Sciences, University of Copenhagen, Denmark.
E-mail: pkm@life.ku.dk

KEYWORDS

comparative map • genetic marker • linkage map • microsatellite • pig • positional quantitative trait loci mining

ABSTRACT

A total of 10 882 porcine microsatellite repeats were identified in genomic shotgun sequences from the Sino-Danish Pig Genome Sequencing Consortium (http://www.piggenome.dk). Of these, 4528 microsatellites were placed on a pig–human comparative map by blast analysis of porcine sequences against the human genome (blast cut-off threshold =1 × 10⁻⁵). All microsatellite sequences placed on the comparative map are accessible at http://www.animalgenome.org/QTLdb/pig.html. These sequences increase the number of identified microsatellites in the porcine genome by several orders of magnitude. They are a new resource of microsatellite sequences for generating markers to be used in linkage studies and in fine mapping and positional cloning of quantitative trait loci.

Accepted for publication 26 March 2007

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1365-2052.2007.01609.x About DOI