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Wiley InterScience | ||||||||||||
  FEMS Microbiology LettersVolume 276 Issue 2, Pages 202 - 206 Published Online: 19 Oct 2007 © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved Published on behalf of the Federation of European Microbiological Societies
Abstract | References | Full Text: HTML, PDF (Size: 173K) | Supporting Information | Related Articles | Citation Tracking  RESEARCH LETTER Global gene expression profiling of wild type and lysC knockout Escherichia coli W3110 Copyright © 2007 Federation of European Microbiological Societies KEYWORDS
Escherichia coli
• gene knockout •
lysC
• lysine starvation • microarray ABSTRACTAspartokinase III, encoded by lysC, is responsible for the first step of lysine biosynthesis in Escherichia coli. In this study, a lysC knockout E. coli W3110 strain was generated to study the differential gene expression profiles of wild type and lysC knockout strains. Several significant changes were observed, including biosynthesis of lysine, oxaloacetate, α-ketoglutarate and glutamate genes. Genes related to transporters and heat shock proteins were also affected by lysC knockout. The results indicated that the lysC knockout strain exhibited some phenomena similar to lysine starvation. The data generated by this study further clarify the systematic role of lysC in lysine biosynthesis. Received 22 June 2007; accepted 30 August 2007. | 
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