|
If you are seeing this message, you may be experiencing temporary network problems. Please wait a few minutes and refresh the page. If the problem persists, you may wish to report it to your local Network Manager.
It is also possible that your web browser is not configured or not able to display style sheets. In this case, although the visual presentation will be degraded, the site should continue to be functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise these problems.
Wiley InterScience | ||||||||||
  CytopathologyVolume 18 Issue 2, Pages 87 - 95 Published Online: 28 Mar 2007 Journal compilation © 2010 Blackwell Publishing Ltd The Official Journal of the British Society for Clinical Cytology and over 20 National affiliated Societies
Abstract | References | Full Text: HTML, PDF (Size: 821K) | Related Articles | Citation Tracking  Detection of urinary bladder cancer with flow cytometry and hexaminolevulinate in urine samples Copyright 2007 The Authors Journal compilation 2007 Blackwell Publishing Ltd KEYWORDS bladder cancer • hexaminolevulinate • photodetection • flow cytometry • urine cytology B. Čunderlíková, R. Wahlqvist, A. Berner, V. Vasovič, T. Warloe, J. M. Nesland and Q. Peng Detection of urinary bladder cancer with flow cytometry and hexaminolevulinate in urine samples ABSTRACTObjectives: Urinary bladder urothelial carcinoma is diagnosed by a combination of cystoscopy and biopsy, with cytology as a valuable additional technique. The accuracy of cytological diagnosis depends on the experience of the cytologist and can inevitably vary from one cytologist to another. There is a need for an easy, reliable and objective diagnostic method. In the present study a new method was designed for the detection of bladder cancer cells in urine. Methods: Flow cytometry was utilized to detect protoporphyrin IX in an artificial model consisting of normal urinary bladder transitional epithelial cells (NBECs) from healthy volunteers' urine and an established human urinary bladder carcinoma cell line, TCCSUP, after incubation with hexaminolevulinate (HAL). In addition, urine samples from 19 patients with histopathologically confirmed superficial bladder cancer were examined. Results: Incubation of NBECs or TCCSUP cells with HAL for 1 hour resulted in production of protoporphyrin IX only in the TCCSUP cells. Incubation of a mixture of NBECs and TCCSUP cells with HAL gave rise to a separated subpopulation of cells with protoporphyrin IX fluorescence. After cell sorting by flow cytometry the protoporphyrin IX-containing subpopulation of cells was confirmed as TCCSUP cells on cytological examination. It was possible to detect 5% TCCSUP cells in the mixture of NBECs/TCCSUP cells. To test the feasibility of the method in clinica diagnosis, urine samples from patients with bladder cancer were also measured with comparable, although preliminary and limited, results to those of cytological examination. Conclusions: The preliminary results show that the technique may be feasible for the detection of bladder cancer cells in urine with possible advantages of simplicity, reliability and objectivity. Accepted for publication 24 October 2006 | 
|
Professor Harald zur Hausen is a 2008 Nobel Laureate in Medicine or Physiology. He was recognized "for his discovery of human papilloma viruses causing cervical cancer". Zur Hausen serves as Editor-in-Chief of the 
