Preliminary investigations on the standardisation and quality control for the determination of γ-glutamyltranspeptidase activity in seminal plasma

doi:10.1111/j.1439-0272.2006.00749.x

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Wiley InterScience

Andrologia

Volume 39 Issue 1, Pages 1 - 6

Published Online: 2 Jan 2007

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ORIGINAL ARTICLE

Preliminary investigations on the standardisation and quality control for the determination of γ-glutamyltranspeptidase activity in seminal plasma

F. Chen ^1,2 , J.-C. Lu ^1,3 , H. R. Xu ^1,4 , Y.-F. Huang ¹ & N.-Q. Lu ⁵

1 Department of Reproduction & Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China;
2 Department of Microbiology and Immunology, Basic Medical College of Nanjing Medical University, Nanjing, China;
3 Department of Laboratory Science, Nanjing Hospital, Jiangsu Corps, the Armed Police Force, PLA, Nanjing, China;
4 Life and Science College, Nanjing Normal University, Nanjing, China;
5 Jiangsu Family Planning Research Institute, Nanjing, China

Correspondence to Prof. Yu-Feng Huang and Dr Jin-Chun Lu, Department of Reproduction & Genetics, Nanjing General Hospital, PLA, 305 East Zhongshan Road, Nanjing 210002, China.
Tel.: +86 25 5299 9865;
Fax: +86 25 8653 5139;
E-mail: jclu@jlonline.com

KEYWORDS

Centrifugation velocity • freezing–thawing • γ-glutamyltranspeptidase • quality control • standardisation

Summary

The present study was designed to assess the effects of centrifugation velocity, standing time after dilution, freezing–thawing and chymotrypsin on the determination of γ-glutamyltranspeptidase (γ-GT) activities in seminal plasma, and to establish an instruction for the standardisation and quality control for the determination of γ-GT within the same laboratory and among different laboratories. The γ-GT level and sperm concentration of each of 72 samples of seminal plasma obtained by centrifugation at 1000 g for 10 min or 3000 g for 15 min were assayed. In addition, γ-GT activities in diluted seminal plasma with different standing time and in samples with or without chymotrypsin were measured. The results showed that there was a significant difference of γ-GT levels in seminal plasma obtained by centrifugation at different velocities (P < 0.001), and that γ-GT activities in seminal plasma measured after standing for 30 min after dilution were notably lower than those measured immediately after dilution (P < 0.001). However, the data indicated that both chymotrypsin and freezing–thawing had no apparent effect on the determination of seminal γ-GT. In conclusion, standing time after dilution and centrifugation velocity should be standardised, and frozen seminal plasma could serve as quality control products for the determination of γ-GT activity among different laboratories.