Validation of an ELISA for the diagnosis of recent Campylobacter infections in Guillain–Barré and reactive arthritis patients

doi:10.1111/j.1469-0691.2007.01765.x

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Wiley InterScience

Clinical Microbiology and Infection

Volume 13 Issue 9, Pages 915 - 922

Published Online: 30 Jun 2007

Published on behalf of the European Society of Clinical Microbiology and Infectious Diseases

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ORIGINAL ARTICLE

Validation of an ELISA for the diagnosis of recent Campylobacter infections in Guillain–Barré and reactive arthritis patients

C. W. Ang ^1,2 , K. Krogfelt ³ , P. Herbrink ⁴ , J. Keijser ⁴ , W. van Pelt ⁵ , T. Dalby ³ , M. Kuijf ⁶ , B. C. Jacobs ⁶ , M. P. Bergman ⁷ , P. Schiellerup ³ and C. E. Visser ^1,2

¹ Department of Medical Microbiology, Academic Medical Center, Amsterdam , ² Department of Medical Microbiology, Reinier de Graaf Gasthuis, Delft, The Netherlands , ³ Unit of Gastrointestinal Infections, Statens Serum Institut, Copenhagen, Denmark , ⁴ Department of Medical Immunology, Reinier de Graaf Gasthuis, Delft , ⁵ Centre for Infectious Diseases Epidemiology, Bilthoven , ⁶ Departments of Neurology & Immunology and ⁷ Department of Medical Microbiology & Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands

Corresponding author and reprint requests: C. W. Ang, Department of Medical Microbiology, AMC, PO Box 22660, 1100 DD Amsterdam, The Netherlands
E-mail: w.ang@amc.uva.nl

KEYWORDS

Arthritis • Campylobacter infections • diagnosis • ELISA • Guillain–Barré syndrome • joint pain

ABSTRACT

Weeks or months following Campylobacter infection, a small proportion of infected individuals develop Guillain–Barré syndrome (GBS) or reactive arthritis (ReA). Stool culture for Campylobacter is often negative in these patients, and serology is therefore the method of choice for diagnosing a recent infection with Campylobacter. This study developed a capture ELISA system to detect anti-Campylobacter IgA and IgM antibodies indicative of a recent infection. The sensitivity of the assay was 82.0% in uncomplicated Campylobacter enteritis patients, 96.2% in GBS patients who were culture-positive for Campylobacter, and 93.1% in culture-positive ReA patients, with a specificity of 93.0%. The assay allows identification of Campylobacter infection in patients with post-infectious neurological and rheumatological complications.

Original Submission: 22 December 2006; Revised Submission: 3 March 2007; Accepted: 5 April 2007

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1469-0691.2007.01765.x About DOI