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Wiley InterScience | |||||||||
  The Plant JournalVolume 45 Issue 4, Pages 684 - 694 Published Online: 26 Jan 2006 Journal compilation © 2010 Blackwell Publishing Ltd and the Society for Experimental Biology Published in association with the Society for Experimental Biology
Abstract | References | Full Text: HTML, PDF (Size: 244K) | Related Articles | Citation Tracking  TECHNIQUES FOR MOLECULAR ANALYSIS TILLING: practical single-nucleotide mutation discovery Copyright 2006 The Authors Journal compilation 2006 Blackwell Publishing Ltd KEYWORDS mutagenesis • polymorphism detection • mutation discovery • mismatch cleavage • gel electrophoresis ABSTRACTIn the post-genomic sequencing era, an expanding portfolio of genomic technologies has been applied to the study of gene function. Reverse genetics approaches that provide targeted inactivation of genes identified by sequence analysis include TILLING (for Targeting Local Lesions IN Genomes). TILLING searches the genomes of mutagenized organisms for mutations in a chosen gene, typically single base-pair substitutions. This review covers practical aspects of the technology, ranging from building the mutagenized population to mutation discovery, and discusses possible improvements to current protocols and the impact of new genomic methods for mutation discovery in relation to the future of the TILLING approach. Received 5 October 2005; revised 27 November 2005; accepted 29 November 2005. | 
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