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Wiley InterScience | |||||||
  Physiologia PlantarumVolume 126 Issue 1, Pages 90 - 96 Published Online: 16 Jan 2006 Copyright © Physiologia Plantarum 2010 Published on behalf of the Scandinavian Society for Plant Physiology
Abstract | References | Full Text: HTML, PDF (Size: 2555K) | Related Articles | Citation Tracking  Review Profiling lipid changes in plant response to low temperatures Edited by V. Hurry Copyright Physiologia Plantarum, 2006 ABSTRACTChanges in membrane lipid composition play multiple roles in plant adaptation and survival in the face of chilling and freezing damage. An electrospray ionization tandem mass spectrometry (ESI-MS/MS)-based approach has been used to quantitatively profile membrane lipid molecular species in plant response to low temperatures. This method involves the direct infusion of unfractionated lipid extracts into a mass spectrometer in the precursor and neutral loss scanning modes to identify and quantify lipid species. The profiling analysis reveals significant and distinct lipid changes during cold acclimation and freezing. Comparative profiling of wildtype and mutants provides information about the metabolic and cellular functions of specific phospholipase D genes and enzymes. Received 22 July 2005; revised 10 October 2005 | 
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