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PRIMER NOTE
Microsatellite DNA markers for Plasmopara viticola, the causal agent of downy mildew of grapes
F. DELMOTTE*, W. J. CHEN*, S. RICHARD-CERVERA*, C. GREIF, D. PAPURA*, X. GIRESSE*, G. MONDOR-GENSON and M. F. CORIO-COSTET*
  *UMR Santé Végétale 1065 (INRA-ENITA), Centre de Recherches INRA Bordeaux Aquitaine, BP 81, 33883 Villenave d'Ornon cedex, France,   UMR Santé de la vigne et qualité du vin (INRA-ULP), Centre de Recherches INRA de Colmar, 28 rue de Herrlisheim, BP 507, 68021 Colmar cedex, France,   CBGP, Campus International de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez cedex, France
Correspondence: F. Delmotte, Fax: 33557122651; E-mail: francois.delmotte@bordeaux.inra.fr
Copyright © 2006 Blackwell Publishing Ltd
KEYWORDS
enriched library • grape downy mildew • microsatellite • Plasmopara viticola

Abstract

AbstractAcknowledgementsReferences

Microsatellite loci were isolated from Plasmopara viticola (Oomycetes), the causal agent of downy mildew of grape, one of the most damaging fungal diseases of grapevine worldwide. Seven polymorphic loci were obtained from an enriched partial genomic library. A low genetic diversity was observed at all loci, with a mean observed allele number of 3.75 and an observed heterozygosity ranging from 0.074 to 0.547. Cross-amplification tests on three closely related taxa indicated that two loci could be used in other Oomycetes species. These microsatellite loci were proved to be useful for population genetic analysis.


Received 15 October 2005; revision accepted 4 November 2005

DIGITAL OBJECT IDENTIFIER (DOI)
10.1111/j.1471-8286.2005.01240.x About DOI

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