Influence of long-term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi

doi:10.1111/j.1469-8137.2006.01793.x

If you are seeing this message, you may be experiencing temporary network problems. Please wait a few minutes and refresh the page. If the problem persists, you may wish to report it to your local Network Manager.

It is also possible that your web browser is not configured or not able to display style sheets. In this case, although the visual presentation will be degraded, the site should continue to be functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise these problems.

Wiley InterScience

New Phytologist

Volume 172 Issue 1, Pages 149 - 158

Published Online: 5 Jun 2006

Published on behalf of the New Phytologist Trust

Go to New Phytologist Trust

< Previous Abstract | Next Abstract >

Save Article to My Profile Download Citation Request Permissions

Abstract | References | Full Text: HTML, PDF (Size: 259K) | Related Articles | Citation Tracking

Influence of long-term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi

Brigitte A. Bastias ¹ , Zhihong Xu ² and John W. G. Cairney ¹

¹ Centre for Plant and Food Science, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South DC and NSW 1797, Australia; ² Centre for Forestry and Horticultural Research and Faculty of Environmental Sciences, Griffith University, Nathan, Queensland 4111, Australia

Author for correspondence: John Cairney Tel: +61 29685 9903 Fax: +61 29685 9915 Email: j.cairney@uws.edu.au

KEYWORDS

ectomycorrhizal fungi • hyphal ingrowth bags • denaturing gradient gel electrophoresis (DGGE) • soilborne mycelia • repeated prescribed burning

New Phytologist (2006) 172: 149–158

doi: 10.1111/j.1469-8137.2006.01793.x

ABSTRACT

•
To demonstrate the efficacy of direct DNA extraction from hyphal ingrowth bags for community profiling of ectomycorrhizal (ECM) mycelia in soil, we applied the method to investigate the influence of long-term repeated prescribed burning on an ECM fungal community.
•
DNA was extracted from hyphal ingrowth bags buried in forest plots that received different prescribed burning treatments for 30 yr, and denaturing gradient gel electrophoresis (DGGE) profiles of partial fungal rDNA internal transcribed spacer (ITS) regions were compared. Restriction fragment length polymorphism (RFLP) and sequence analyses were also used to compare clone assemblages between the treatments.
•
The majority of sequences derived from the ingrowth bags were apparently those of ECM fungi. DGGE profiles for biennially burned plots were significantly different from those of quadrennially burned and unburned control plots. Analysis of clone assemblages indicated that this reflected altered ECM fungal community composition.
•
The results indicate that hyphal ingrowth bags represent a useful method for investigation of ECM mycelial communities, and that frequent long-term prescribed burning can influence below-ground ECM fungal communities.

Received: 22 March 2006 Accepted: 21 April 2006

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1469-8137.2006.01793.x About DOI