PHYLOGENY OF THE HYDRODICTYACEAE (CHLOROPHYCEAE): INFERENCES FROM rDNA DATA1

doi:10.1111/j.1529-8817.2005.00129.x

If you are seeing this message, you may be experiencing temporary network problems. Please wait a few minutes and refresh the page. If the problem persists, you may wish to report it to your local Network Manager.

It is also possible that your web browser is not configured or not able to display style sheets. In this case, although the visual presentation will be degraded, the site should continue to be functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise these problems.

Systems Maintenance, Monday, 15th February 2010

Wiley InterScience

Journal of Phycology

Volume 41 Issue 5, Pages 1039 - 1054

Published Online: 13 Sep 2005

Published on behalf of the Phycological Society of America

Go to Society Site

< Previous Abstract | Next Abstract >

Save Article to My Profile Download Citation Request Permissions

Abstract | References | Full Text: HTML, PDF (Size: 1419K) | Related Articles | Citation Tracking

PHYLOGENY OF THE HYDRODICTYACEAE (CHLOROPHYCEAE): INFERENCES FROM rDNA DATA¹

Mark Buchheim ^a ² , Julie Buchheim ^a , Tracy Carlson ^a , Anke Braband ^b , Dominik Hepperle ^c , Lothar Krienitz ^d , Matthias Wolf ^e , and Eberhard Hegewald ^f

^a Department of Biological Science and the Mervin Bovaird Institute for Molecular Biology and Biotechnology, The University of Tulsa, 600 South College Avenue, Tulsa, Oklahoma 74104, USA
^b Humboldt-Universität zu Berlin, Institut für Biologie/Vergleichende Zoologie, Philippstr. 13, D-10115 Berlin, Germany
^c Dorfstr. 20, D-18249 Klein Raden and Leibniz Institute of Freshwater Ecology & Inland Fisheries, D-16775 Stechlin-Neuglobsow, Germany
^d Leibniz Institute of Freshwater Ecology & Inland Fisheries, D-16775 Stechlin-Neuglobsow, Germany
^e Leibniz Institute of Freshwater Ecology & Inland Fisheries, D-16775 Stechlin-Neuglobsow and Department of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany
^f Institute of Chemistry and Dynamics of the Geosphere, Institute III: Phytosphere, Research Centre Jülich, D-52425 Jülich, Germany

¹Received 15 October 2004. Accepted 30 May 2005.

²Author for correspondence: e-mail mark-buchheim@utulsa.edu.

KEYWORDS

18S rDNA • 26S rDNA • Chlorotetraedron • comb. nov. • gen. nov. • Hydrodictyon • ITS-2 • molecular systematics • Monactinus • Parapediastrum • Pediastrum • phylogeny • Pseudopediastrum • Sorastrum • Stauridium • Tetraedron

ABSTRACT

The hydrodictyacean green algal lineage has been the focus of much research due to the fossil record of at least some members, their ornamented cell walls, and their distinctive reproductive strategies. The phylogeny of the family was, until recently, exclusively morphology based. This investigation examines hydrodictyacean isolates from several culture collections, focusing on sequences from ribosomal data: 18S rDNA, 26S rDNA (partial), and internal transcribed spacer (ITS)-2 data. Results from phylogenetic analyses of independent and combined data matrices support the Hydrodictyaceae as a monophyletic lineage that includes isolates of Chlorotetraedron, Hydrodictyon, Pediastrum, Sorastrum, and Tetraedron. Phylogenetic analyses of rDNA data indicate that the three-dimensional coenobium of Hydrodictyon is evolutionarily distinct from the three-dimensional coenobium of Sorastrum. The more robust aspects of the ITS-2 data corroborate the 18S+26S rDNA topology and provide a structural autapomorphy for the Hydrodictyaceae and Neochloridaceae, that is, an abridgment of helix IV in the secondary structure. The rDNA data do not support monophyly of Pediastrum but rather suggest the existence of four additional hydrodictyacean genera: Monactinus, Parapediastrum, Pseudopediastrum, and Stauridium.