The Arabidopsis CDC25 induces a short cell length when overexpressed in fission yeast: evidence for cell cycle function

doi:10.1111/j.1469-8137.2004.01288.x

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Wiley InterScience

New Phytologist

Volume 165 Issue 2, Pages 425 - 428

Published Online: 15 Dec 2004

Published on behalf of the New Phytologist Trust

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The Arabidopsis CDC25 induces a short cell length when overexpressed in fission yeast: evidence for cell cycle function

D. A. Sorrell ^1,2 , D. Chrimes ^1,3 , J. R. Dickinson ¹ , H. J. Rogers ¹ and D. Francis ¹

¹ School of Biosciences, Cardiff University, PO Box 915, Cardiff, CF10 3TL, UK; ² Hannah Research Institute, Ayr, KA6 5HL, UK; ³ School of Biological Sciences, Manchester University, Oxford Road, Manchester, M13 9PT, UK

Author for correspondence: D. Francis Tel: +44 29 20875086 Fax: +44 29 20874305 Email: francisd@cf.ac.uk

KEYWORDS

Arabidopsis thaliana • CDC25 • cell cycle • cell size • fission yeast

New Phytologist (2004) doi: 10.1111/j.1469-8137.2004.01288.x

©New Phytologist (2004)

Summary

•
The putative mitotic inducer gene, Arath;CDC25 cloned in Arabidopsis thaliana, was screened for cell cycle function by overexpressing it in Schizosaccharomyces pombe (fission yeast). The expression pattern of Arath;CDC25 was also examined in different tissues of A. thaliana.
•
Fission yeast was transformed with plasmids pREP1 and pREP81 with the Arath;CDC25 gene under the control of the thiamine-repressible nmt promoter. Using reverse transcription-polymerase chain reaction (RT-PCR), the expression of Arath;CDC25 was examined in seedlings, flower buds, mature leaves and stems of A. thaliana; actin (ACT2) was used as a control.
•
In three independent transformants of fission yeast, cultured in the absence of thiamine (T), pREP1::Arath;CDC25 induced a highly significant reduction in mitotic cell length compared with wild type, pREP::Arath;CDC25 +T, and empty vector (pREP1 ± T). The extent of cell shortening was greater using the stronger pREP1 compared with the weaker pREP81. However, Arath;CDC25 was expressed at low levels in all tissues examined.
•
The data indicate that Arath;CDC25 can function as a mitotic accelerator in fission yeast. However, unlike other plant cell cycle genes, expression of Arath;CDC25 was not enhanced in rapidly dividing compared with non-proliferative Arabidopsis tissues.