Comparison of lignin deposition in three ectopic lignification mutants

doi:10.1111/j.1469-8137.2005.01496.x

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Wiley InterScience

New Phytologist

Volume 168 Issue 1, Pages 123 - 140

Published Online: 1 Jul 2005

Published on behalf of the New Phytologist Trust

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Comparison of lignin deposition in three ectopic lignification mutants

Louisa A. Rogers ^1,3,6 , Christian Dubos ^1,4,6 , Christine Surman ¹ , Janet Willment ^1,5 , Ian F. Cullis ² , Shawn D. Mansfield ² and Malcolm M. Campbell ^1,4

¹ Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK, ² Department of Wood Science, University of British Columbia, 4030–2424 Main Mall, Vancouver, BC, Canada V6T 1Z4; ³ Present address: CAB International Publishing, Wallingford OX10 8DE, UK, ⁴ Present address: Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2; ⁵ Present address: Institute of Infectious Disease and Molecular Medicine, Faculty Of Health Sciences, University of Cape Town, Observatory 7925, Cape Town, South Africa; ⁶ These authors contributed equally to this manuscript

Author for correspondence: Malcolm M. Campbell Tel: +1 416 9460817 Fax: +1 416 9785878 Email: campbell@botany.utoronto.ca

KEYWORDS

Arabidopsis • ectopic lignification • lignins • phenylpropanoid • transcription

New Phytologist (2005) doi: 10.1111/j.1469-8137.2005.01496.x

©New Phytologist (2005)

Summary

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The Arabidopsis thaliana mutants de-etiolated3 (det3), pom-pom1 (pom1) and ectopic lignification1 (eli1) all deposit lignins in cells where these polymers would not normally be found. Comparison of these mutants provides an opportunity to determine if the shared mutant phenotype arose by perturbing a common regulatory mechanism in each of the mutants.
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The mutants were compared using a combination of genetics, histochemistry, chemical profiling, transcript profiling using both Northern blots and microarrays, and bioinformatics.
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The subset of cells that ectopically lignified was shared between all three mutants, but clear differences in cell wall chemistry were evident between the mutants. Northern blot analysis of lignin biosynthetic genes over diurnal and circadian cycles revealed that transcript abundance of several key genes was clearly altered in all three mutants. Microarray analysis suggests that changes in the expression of specific members of the R2R3-MYB and Dof transcription factor families may contribute to the ectopic lignification phenotypes.
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This comparative analysis provides a suite of hypotheses that can be tested to examine the control of lignin biosynthesis.