Ethylene-binding activity, gene expression levels, and receptor system output for ethylene receptor family members from Arabidopsis and tomato†

doi:10.1111/j.1365-313X.2004.02331.x

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Wiley InterScience

The Plant Journal

Volume 41 Issue 5, Pages 651 - 659

Published Online: 17 Jan 2005

Published in association with the Society for Experimental Biology

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Ethylene-binding activity, gene expression levels, and receptor system output for ethylene receptor family members from Arabidopsis and tomato^†

Ronan C. O'Malley ¹ , Fernando I. Rodriguez ^1,‡ , Jeffrey J. Esch ¹ , Brad M. Binder ¹ , Philip O'Donnell ² , Harry J. Klee ² and Anthony B. Bleecker ^1,3,*

¹ Department of Botany, University of Wisconsin, 430 Lincoln Drive, Madison, WI 53706, USA ,
² Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA , and
³ Laboratory of Genetics, University of Wisconsin, 430 Lincoln Drive, Madison, WI 53706, USA

Correspondence to ^* (fax +608 262 7509; e-mail bleecker@wisc.edu).

^‡Present address: Department of Biochemistry, Biotechnology Center 2302, 425 Henry Mall, Madison, WI 53706, USA.

^† Just prior to print publication of this article, our colleague and friend, Tony Bleecker, passed away on January 30, 2005. The authors wish to dedicate this paper to the memory of Tony and also to Jeff Esch, who passed away last year.

KEYWORDS

ethylene receptor • signal transduction • plant hormone • membrane-associated receptor • ETR1

ABSTRACT

Ethylene signaling in plants is mediated by a family of ethylene receptors related to bacterial two-component regulators. Expression in yeast of ethylene-binding domains from the five receptor isoforms from Arabidopsis thaliana and five-receptor isoforms from tomato confirmed that all members of the family are capable of high-affinity ethylene-binding activity. All receptor isoforms displayed a similar level of ethylene binding on a per unit protein basis, while members of both subfamily I and subfamily II from Arabidopsis showed similar slow-release kinetics for ethylene. Quantification of receptor-isoform mRNA levels in receptor-deficient Arabidopsis lines indicated a direct correlation between total message level and total ethylene-binding activity in planta. Increased expression of remaining receptor isoforms in receptor-deficient lines tended to compensate for missing receptors at the level of mRNA expression and ethylene-binding activity, but not at the level of receptor signaling, consistent with specialized roles for family members in receptor signal output.

Received 4 November 2004; accepted 18 November 2004.

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1365-313X.2004.02331.x About DOI