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Wiley InterScience | ||||||||||||||||
  Insect Molecular BiologyVolume 14 Issue 2, Pages 145 - 150 Published Online: 23 Feb 2005 Journal compilation © 2009 Royal Entomological Society
Abstract | References | Full Text: HTML, PDF (Size: 247K) | Related Articles | Citation Tracking  Genetic variation in the sand fly salivary protein, SP-15, a potential vaccine candidate against Leishmania major Copyright © 2005 The Royal Entomological Society KEYWORDS SP-15 • sandflies • saliva • Leishmaniasis • vaccines Abstract
SP-15 is a sandfly salivary protein that provides strong protection against cutaneous leishmaniasis, caused by Leishmania major, and has been proposed as a potential vaccine against this disease. To investigate possible antigenic variation in this protein, we examined genetic polymorphism of SP-15 in 100 Phlebotomus papatasi sandflies, from a natural population from Sudan and four laboratory colonies from Egypt, Jordan, Israel and Saudi Arabia. We found that although many variants of SP-15 may be found in nature, differences among them are minimal (mean ± SD pairwise differences = 1.69 ± 0.83% for forty nucleotide sequences and 3.06 ± 1.13% for thirty amino acid sequence variants). Analysis of proportions of synonymous and non-synonymous substitutions indicated that SP-15 is not under diversifying selection. Our results suggest that a vaccine based on SP-15 protein should result in a uniform immune response. Received 9 February 2004; accepted after revision 13 October 2004. | 
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