Modulation of Anopheles gambiae gene expression in response to o'nyong-nyong virus infection

doi:10.1111/j.1365-2583.2005.00578.x

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Wiley InterScience

Insect Molecular Biology

Volume 14 Issue 5, Pages 475 - 481

Published Online: 24 Aug 2005

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Modulation of Anopheles gambiae gene expression in response to o'nyong-nyong virus infection

C. Sim*, Y. S. Hong†, D. L. Vanlandingham‡, B. W. Harker*, G. K. Christophides§, F. C. Kafatos§, S. Higgs‡ and F. H. Collins*

*Center for Tropical Disease Research and Training, University of Notre Dame, Notre Dame, USA, †Department of Tropical Medicine, Tulane University, New Orleans, LA, USA, ‡Pathology Department, University of Texas Medical Branch, Galveston, TX, USA, §European Molecular Biology Laboratory, Heidelberg, Germany

Correspondence: Frank H. Collins, Center for Tropical Disease Research and Training, University of Notre Dame, Notre Dame, IN 46556, USA. Tel.: +1 574 631 3241; fax: +1 574 631 3996; e-mail: frank@nd.edu

KEYWORDS

Anopheles gambiae • o'nyong-nyong virus • gene expression • cDNA microarray

Abstract

To determine if gene expression of An. gambiae is modulated in response to o'nyong-nyong virus (ONNV) infection, we utilized cDNA microarrays including about 20 000 cDNAs. Gene expression levels of ONNV-infected female mosquitoes were compared to that of the uninfected control females harvested at 14 days postinfection. In response to ONNV infection, expression levels of 18 genes were significantly modulated, being at least two-fold up- or down-regulated. Quantitative real-time PCR analysis (qRT-PCR) further substantiated the differential expression of six of these genes in response to ONNV infection. These genes have similarity to a putative heat shock protein 70, DAN4, agglutinin attachment subunit, elongation factor 1 alpha and ribosomal protein L35. One gene, with sequence similarity to mitochondrial ribosomal protein L7, was down-regulated in infected mosquitoes. The expression levels and annotation of the differentially expressed genes are discussed in the context of host/virus interaction including host translation/replication factors, and intracellular transport pathways.

doi: 10.1111/j.1365-2583.2005.00578.x

Received 28 December 2004; accepted after revision 24 March 2005.

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1365-2583.2005.00578.x About DOI