|
If you are seeing this message, you may be experiencing temporary network problems. Please wait a few minutes and refresh the page. If the problem persists, you may wish to report it to your local Network Manager.
It is also possible that your web browser is not configured or not able to display style sheets. In this case, although the visual presentation will be degraded, the site should continue to be functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise these problems.
Wiley InterScience | ||||||||||||||
  Journal of Field OrnithologyVolume 77 Issue 2, Pages 229 - 232 Published Online: 15 May 2006 Journal compilation © 2009 Association of Field Ornithologists Published on behalf of the Association of Field Ornithologists, Inc
Abstract | References | Full Text: HTML, PDF (Size: 67K) | Related Articles | Citation Tracking  Sex determination of Pohnpei Micronesian Kingfishers using morphological and molecular genetic techniques Copyright Journal compilation ©2006 Association of Field Ornithologists KEYWORDS
Halcyon cinnamomina
• Micronesian Kingfisher • molecular sex determination • morphometrics • plumage •
Todiramphus cinnamominus
ABSTRACTABSTRACT. Conservation-oriented studies of Micronesian Kingfishers (Todiramphus cinnamominus) have been hindered by a lack of basic natural history information, despite the status of the Guam subspecies (T. c. cinnamominus) as one of the most endangered species in the world. We used tissue samples and morphometric measures from museum specimens and wild-captured Pohnpei Micronesian Kingfishers (T. c. reichenbachii) to develop methods for sex determination. We present a modified molecular protocol and a discriminant function that yields the probability that a particular individual is male or female. Our results revealed that females were significantly larger than males, and the discriminant function correctly predicted sex in 73% (30/41) of the individuals. The sex of 86% (18/21) of individuals was correctly assigned when a moderate reliability threshold was set. Sex determination using molecular genetic techniques was more reliable than methods based on morphology. Our results will facilitate recovery efforts for the critically endangered Guam Micronesian Kingfisher and provide a basis for sex determination in the 11 other endangered congeners in the Pacific Basin. ABSTRACTEstudios dirigidos a conservar a Todiramphus cinnamominus se han visto ausentes de conocimientos básicos sobre la historia natural del ave, aún tomándose en consideración que la subespecie de Guam es una de las formas más amenazadas con desaparecer. Utilizamos datos morfométricos de pieles en museo y muestras de tejido de la subespecie de Pohnpei (T. c. reichenbachii) para desarrollar un método útil para determinar el sexo de las aves. Presentamos un protocolo molecular modificado y una función de análisis discriminativo que ofrece una buena probabilidad para identificar los sexos. Nuestro estudio indica que las hembras son significativamente más grandes que los machos y el análisis estadístico predijo el sexo en el 73% (30/41) de los individuos. El sexo del 86% (18/21) de los individuos fue acertado cuando se preparó un umbral moderado de confiabilidad. El determinar el sexo utilizando genética molecular fue más confiable que usando morfometría. Nuestros resultados facilitarán los esfuerzos por recobrar a esta especie que esta criticamente amenazada y proveerá de una base sólida para determinar el sexo de 11 congéneres también amenazados que habitan en la Cuenca del Pacífico. Received 1 December 2005; accepted 8 February 2006 | 
|
| ||||||||||||
Best Student Paper Award |  |
The Association of Field Ornithologists gives an award for the best paper published by a student in each volume of the Journal of Field Ornithology. | |
 |
