Effect of neonatal exposure to diethylstilbestrol on testicular gene expression in adult mouse: comprehensive analysis with cDNA subtraction method

doi:10.1046/j.0105-6263.2003.00460.x

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Wiley InterScience

International Journal of Andrology

Volume 27 Issue 2, Pages 115 - 122

Published Online: 5 Mar 2004

The Official Journal of the European Academy of Andrology

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Effect of neonatal exposure to diethylstilbestrol on testicular gene expression in adult mouse: comprehensive analysis with cDNA subtraction method

Yoshiharu Matsuno*§§, Tetsuya Adachi*†§§, Kyu-Bom Koh*, Hideki Fukata‡, Atsushi Sugimura§, Kenichi Sakurai¶, Takako Shibayama*, Taisen Iguchi**††, Masatoshi Komiyama*‡‡ and Chisato Mori*††

*Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan , †Center for Research and Development of Bioresources, Research Institute for Advanced Science and Technology, Osaka Prefecture, University, Sakai 599-8531, Japan , ‡Department of Environmental Medical Science (SRL), Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan , §Advanced Medical Technology Research and Development Center, JCR Pharmaceuticals Co. Ltd., Kobe 651-2241, Japan , ¶Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan , **Department of Bioenvironmental Research, Center for Integrative Bioscience, Okazaki National Research Institutes, Okazaki 444-8585, Japan , ††Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Kawaguchi 332-0012, Japan , and ‡‡Center for Environment, Health and Field Sciences, Chiba University, Kashiwa, 277-0882, Japan

Correspondence to Chisato Mori MD D Med Sci, Department of Bioenvironmental Medicine (A3), Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan. E-mail: cmori@faculty.chiba-u.jp

§§Authors who contributed equally to this study.

KEYWORDS

cDNA subtraction • diethylstilbestrol • oestrogen • mouse • testis

ABSTRACT

In utero or neonatal exposure to high levels of exogenous steroid hormones, such as the potent synthetic diethylstilbestrol (DES), incurs an increased risk of malfunctional male reproduction. In this study, we investigated whether neonatal exposure to DES induces the alteration of mRNA expression in adult mouse testis. Using a cDNA subtraction method, we isolated seven gene clones whose expression was changed in neonatally DES-treated mouse testis. Northern blot analysis revealed that five up-regulated genes (AF326230, AF356521, AK004975, AK006136 and BM237156) and two down-regulated genes (AK017044, AK017130) were predominantly expressed in testes of 8-week-old mice. Moreover, we confirmed that the expression of these seven genes was altered by neonatal DES-exposure using Northern blot analysis. Our results suggest that neonatal exposure to DES leads to the alteration of gene expression in the testis in the long term. These genes might be useful as biological markers of foetal or neonatal exposure to exogenous steroid hormones, such as DES.

Received 11 August 2003; revised 9 October 2003; accepted 14 October 2003

DIGITAL OBJECT IDENTIFIER (DOI)

10.1046/j.0105-6263.2003.00460.x About DOI