|
If you are seeing this message, you may be experiencing temporary network problems. Please wait a few minutes and refresh the page. If the problem persists, you may wish to report it to your local Network Manager.
It is also possible that your web browser is not configured or not able to display style sheets. In this case, although the visual presentation will be degraded, the site should continue to be functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise these problems.
Wiley InterScience | |||||||||
  FEMS Microbiology EcologyVolume 48 Issue 1, Pages 101 - 107 Published Online: 5 Jan 2006 © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved Published on behalf of the Federation of European Microbiological Societies
Abstract | References | Full Text: HTML, PDF (Size: 259K) | Related Articles | Citation Tracking  High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region Copyright 2004 Federation of European Microbiological Societies KEYWORDS
Mesorhizobium
• 16S-rDNA • DAPD • Chickpea • Diversity • Microbial ecology ABSTRACTChickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal. Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations. Received 10 July 2003, Revised 19 November 2003, Accepted 23 December 2003 | 
|
|
