Isolation of two microsatellite markers from BAC clones of the Vf scab resistance region and molecular characterization of scab-resistant accessions in Malus germplasm*

doi:10.1111/j.1439-0523.2004.00973.x

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Wiley InterScience

Plant Breeding

Volume 123 Issue 4, Pages 321 - 326

Published Online: 28 Jun 2008

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Isolation of two microsatellite markers from BAC clones of the Vf scab resistance region and molecular characterization of scab-resistant accessions in Malus germplasm*

B. A. Vinatzer ^1, ³ , A. Patocchi ^2, ⁵ , S. Tartarini ³ , L. Gianfranceschi ⁴ , S. Sansavini ³ C. Gessler ²

¹ Current address: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL-60637, USA ² Plant Pathology group, Institute of Plant Science, Swiss Federal Institute of Technology, Zurich CH-8092, Switzerland ³ Dipartimento di Colture Arboree (DCA), University of Bologna, Bologna I-40126, Italy ⁴ Department of Genetics and Biology of Microorganisms (DGBM), University of Milano, Milano I-20133, Italy

⁵Corresponding author. E-mail: andrea.patocchi@ipw.agrl.ethz.ch

* The first two authors contributed equally to the work.

With 4 figures and 1 table

Communicated by W. E. Weber

KEYWORDS

Venturia inaequalis • bacterial artificial chromosome • simple sequence repeat

Abstract

A polymerase chain reaction (PCR)-based method was developed to isolate microsatellite markers from large-insert genomic DNA clones of bacterial artificial chromosome (BAC) libraries. The method is fast and economic since it does not require subcloning. It was applied to isolate a microsatellite marker from a BAC clone of the chromosomal region containing the apple scab resistance gene Vf. The Vf gene of Malus floribunda 821 is the most widely used source of scab resistance in apple breeding. A second microsatellite was found on the extremity of a BAC clone flanking the Vf locus. The two microsatellites allowed the identification of the presence of the Vf gene in the scab-resistant accessions M. micromalus SA573-3, 'Golden Gem', M. prunifolia 19651 and MA 16 not previously known to carry this gene. They were also used to verify the correctness of the published genealogical tree of the Vf cultivar 'Florina', in which a probable mistake was identified. This analysis shows the importance of genotyping the Vf locus when choosing scab-resistant germplasm as parents in breeding programmes.

Received July 21, 2003/Accepted November 10, 2003

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1439-0523.2004.tb01825.x About DOI