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Wiley InterScience

Acta Crystallographica Section D

Acta Crystallographica Section D

Volume 60 Issue 11, Pages 1958 - 1963

International Union of Crystallography, 2010



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Radiation-damage-induced phasing with anomalous scattering: substructure solution and phasing
Petrus H. Zwart Sankaran Banumathi Miroslawa Dauter Zbigniew Dauter
Correspondence to Zbigniew Dauter, e-mail: dauter@bnl.gov
Copyright International Union of Crystallography, 2004
KEYWORDS
anomalous scattering • radiation damage • RIP • RIPAS.

ABSTRACT

Substructure-solution and phasing procedures using a combination of anomalous scattering and radiation-damage-induced isomorphous differences have been investigated. The tyrosine residues in thaumatin were iodinated with N-­iodosuccinimide in the crystalline form as well as prior to crystallization. Several data sets were collected from both forms and used for substructure solution and phasing using various protocols, employing anomalous, isomorphous or both these signals. It was shown that combination of the anomalous and isomorphous signals in the form of the RIPAS (radiation-damage-induced phasing with anomalous scattering) strategy is beneficial for both locating the substructure and subsequent phasing.


Received 10 June 2004, accepted 3 September 2004

DIGITAL OBJECT IDENTIFIER (DOI)
10.1107/S0907444904021730 About DOI

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