Chromosome Polymorphism of the Obligate Biotrophic Parasite Plasmodiophora brassicae

doi:10.1046/j.1439-0434.2003.00805.x

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Wiley InterScience

Journal of Phytopathology

Volume 152 Issue 2, Pages 86 - 91

Published Online: 20 Feb 2004

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Chromosome Polymorphism of the Obligate Biotrophic Parasite Plasmodiophora brassicae

H. Graf ¹ , M. Fähling ¹ and J. Siemens ^1,2

Authors' addresses: ¹Institut für Biologie – Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin; ²Technische Universität Dresden, Fachrichtung Biologie, Molekulare Biotechnologie, 01062 Dresden, Germany

Correspondence to Institut für Biologie – Angewandte Genetik, Freie Universität Berlin, Berlin, Germany

KEYWORDS

clubroot • karyotype • chromosome polymorphism • single-spore isolate

Abstract

Electrophoretic karyotypes of different isolates of Plasmodiophora brassicae, the causative agent of clubroot of cabbage, using contour-clamped homogeneous electric field gel electrophoresis (CHEF) revealed chromosome polymorphism in this obligate parasite. Ribosomal RNA (rRNA) genes have been localized on three chromosomes (I, IV and V) of P. brassicae of the 16 chromosomal bands, which can be distinguished for the single-spore isolate 'e₃'. In comparison with this isolate three other single-spore isolates showed chromosome polymorphism by size of chromosomal bands and by hybridization pattern with rRNA gene fragments and other Plasmodiophora-specific DNA fragments.

Received March 14, 2003; accepted November 7, 2003

DIGITAL OBJECT IDENTIFIER (DOI)

10.1046/j.1439-0434.2003.00805.x About DOI