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Wiley InterScience | ||||||||||||||||||
  Plant Biotechnology JournalVolume 2 Issue 6, Pages 495 - 506 Published Online: 24 Aug 2004 Journal compilation © 2010 Blackwell Publishing Ltd Plant Biotechnology Journal is published by Wiley-Blackwell in association with the Society for Experimental Biology (SEB) and the Association of Applied Biologists (AAB).
Abstract | References | Full Text: HTML, PDF (Size: 340K) | Related Articles | Citation Tracking  A transcriptomics resource for wheat functional genomics † Present addresses: Richard W. Beswick is currently at the Department of Haematology, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK; Sophie K. Shepherd is at the University of Reading, Department of Agriculture, Whiteknights, Reading RG6 6AH, UK; David Edwards is at Primary Industries Research Victoria, Department of Primary Industries, La Trobe University, Bundoora, Victoria 3086, Australia; and Michael J. Holdsworth is at the University of Nottingham, School of Biosciences, Division of Agricultural and Environmental Sciences, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK. Copyright © 2004 Blackwell Publishing Ltd KEYWORDS cDNA • EST • microarray • transcriptome •
Triticum aestivum
Summary
Grain development, germination and plant development under abiotic stresses are areas of biology that are of considerable interest to the cereal community. Within the Investigating Gene Function programme we have produced the resources required to investigate alterations in the transcriptome of hexaploid wheat during these developmental processes. We have single pass sequenced the cDNAs of between 700 and 1300 randomly picked clones from each of 35 cDNA libraries representing highly specific stages of grain and plant development. Annotated sequencing results have been stored in a publicly accessible, online database at http://www.cerealsdb.uk.net. Each of the tissue stages used has also been photographed in detail, resulting in a collection of high-quality micrograph images detailing wheat grain development. These images have been collated and annotated in order to produce a web site focused on wheat development (http://www.wheatbp.net/). We have also produced high-density microarrays of a publicly available wheat unigene set based on the 35 cDNA libraries and have completed a number of microarray experiments which validate their quality. Received 10 April 2004; revised 2 June 2004; accepted 2 June 2004. | 
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