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PRIMER NOTE
Isolation and characterization of 11 polymorphic microsatellite loci in collared lizards (Crotaphytus collaris)
DELBERT W. HUTCHISON*, JARED L. STRASBURG, JENNIFER A. BRISSON and SHAWN CUMMINGS
  *Biology Department, Whitman College, Walla Walla, WA, 99362, USA,   Department of Biology, Campus Box 1137, Washington University, St. Louis, MO 63130, USA,   7205 Delta Avenue, St. Louis, MO, 63117, USA
 Correspondence: D. W. Hutchinson. Fax: 509 527 5904; E-mail: hutchidw@whitman.edu
Copyright © 2004 Blackwell Publishing Ltd
KEYWORDS
Crotaphytus collaris • genetic variation • microsatellites • peripheral populations • population structure

Abstract

AbstractAcknowledgementsReferences

We identified 11 polymorphic microsatellite loci in collared lizards (Crotaphytus collaris). Polymorphism assessment in 512 individuals from 52 populations sampled across much of the species distribution revealed a fairly high degree of genetic diversity (six to 20 alleles per locus) and a wide range of average expected heterozygosity values (0.143–0.530). We found no evidence for linkage, very few deviations from HW expectation (two of 572 possible population/locus analyses) and thus no evidence for null alleles. There was a tendency for reduced polymorphism towards the northern periphery.


Received 26 March 2004; revision received 28 May 2004; accepted 28 May 2004

DIGITAL OBJECT IDENTIFIER (DOI)
10.1111/j.1471-8286.2004.00730.x About DOI

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