Design of a New Pulsatile Bioreactor for Tissue Engineered Aortic Heart Valve Formation

doi:10.1046/j.1525-1594.2002.06931_3.x

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Wiley InterScience

Artificial Organs

Volume 26 Issue 8, Pages 710 - 714

Published Online: 26 Jul 2002

Official Peer-Reviewed Journal of the International Federation for Artificial Organs (members of the Federation are: the American Society for Artificial Internal Organs, the European Society for Artificial Organs and the Japanese Society for Artificial Organs), the International Faculty for Artificial Organs, and the International Society for Rotary Blood Pumps.

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Design of a New Pulsatile Bioreactor for Tissue Engineered Aortic Heart Valve Formation

*Kris Dumont, †Jessa Yperman, ‡Erik Verbeken, *Patrick Segers, †Bart Meuris, *Stijn Vandenberghe, †Willem Flameng, and *Pascal R. Verdonck;

* IBiTech, Institute of Biomedical Technology, Ghent University, Gent; † CEHA, Center for Experimental Surgery and Anesthesiology; and ‡ Division for Morphology and Molecular Pathology, Katholieke Universiteit Leuven, Leuven, Belgium

Address correspondence and reprint requests to Kris Dumont, M.Sc.Civ.E., Hydraulics Laboratory, Ghent University, Sint-Pietersnieuwstraat 41 9000 Gent, Belgium. E-mail: kris.dumont@rug.ac.be

KEYWORDS

Pulsatile bioreactor • Tissue Engineering • Aortic Valve • Biocompatibility • Cardiovascular mechanics

Abstract:

Abstract:Evidence has been gathered that biomechanical factors have a significant impact on cell differentiation and behavior in in vitro cell cultures. The aim of this bioreactor is to create a physiological environment in which tissue engineered (TE) aortic valves seeded with human cells can be cultivated during a period of several days. The bioreactor consists of 2 major parts: the left ventricle (LV) and the afterload consisting of a compliance, representing the elastic function of the large arteries, and in series a resistance, mimicking the arterioles and capillaries. The TE aortic valve is placed between the LV and the compliance. With controllable resistance, compliance, stroke volume and frequency, and hydrodynamic conditions can be changed over a wide physiological range. This study resulted in a prototype of a compact pulsatile flow system for the creation of TE aortic valves. In addition a biocompatibility study of the used materials is performed.

Received October 2001; revised February 2002.

DIGITAL OBJECT IDENTIFIER (DOI)

10.1046/j.1525-1594.2002.06931_3.x About DOI