|
If you are seeing this message, you may be experiencing temporary network problems. Please wait a few minutes and refresh the page. If the problem persists, you may wish to report it to your local Network Manager.
It is also possible that your web browser is not configured or not able to display style sheets. In this case, although the visual presentation will be degraded, the site should continue to be functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise these problems.
Wiley InterScience | |||||
  Clinical & Experimental AllergyVolume 32 Issue 2, Pages 287 - 295 Published Online: 8 Apr 2002 © 2010 Blackwell Publishing Ltd The Official Journal of the British Society for Allergy & Clinical Immunology
Abstract | References | Full Text: HTML, PDF (Size: 126K) | Related Articles | Citation Tracking  Effect of formaldehyde on the expression of adhesion molecules in nasal microvascular endothelial cells: the role of formaldehyde in the pathogenesis of sick building syndrome Copyright Blackwell Science Ltd, 2002 KEYWORDS eosinophils • formaldehyde • human nasal microvascular endothelial cells • ICAM‐1 • sick building syndrome • VCAM‐1 ABSTRACTBackgroundFormaldehyde is associated with sick building syndrome (SBS), a set of diffuse and irritative symptoms predominantly involving the eyes and the respiratory tract. However, its pathophysiological mechanism in SBS has not yet been clarified. ObjectiveIn this study we investigated the effect of formaldehyde on the expression of adhesion molecules on human mucosal microvascular endothelial cells (HMMECs). Furthermore, we investigated the effect of formaldehyde on adhesiveness of HMMECs to eosinophils. Materials and methodsHMMECs were incubated with various concentrations of formaldehyde (1 ng/mL−1 µg/mL) for 24 h, and the expressions of intercellular adhesion molecule‐1 (ICAM‐1) and vascular cell adhesion molecule (VCAM‐1) on HMMECs were evaluated by flow cytometry. The change in the expression of ICAM‐1 and VCAM‐1 mRNA was then evaluated by reverse transcriptase polymerase chain reaction. To understand the role of formaldehyde in eosinophilic inflammation of the nasal mucosa, we examined the effects of formaldehyde on the adhesiveness between HMMECs and eosinophils by eosinophil adhesion assay. ResultsFormaldehyde increased the surface expressions of ICAM‐1 and VCAM‐1 on HMMECs. Formaldehyde also induced ICAM‐1 and VCAM‐1 mRNA. In addition, the adhesiveness between HMMECs and eosinophils was also increased by formaldehyde. ConclusionThese in vitro studies suggest that formaldehyde may play a role as the irritant of the nasal mucosa by increasing the expressions of adhesion molecules on HMMECs and by enhancing the adhesiveness between HMMECs and eosinophils. Submitted 6 February 2001; revised 9 July 2001; accepted 20 August 2001 | 
|
