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Wiley InterScience | ||||||||||||||
  Journal of MicroscopyVolume 201 Issue 3, Pages 368 - 376 Published Online: 21 Dec 2001 Journal compilation © 2010 Royal Microscopical Society
Abstract | References | Full Text: HTML, PDF (Size: 480K) | Related Articles | Citation Tracking  High efficiency beam splitter for multifocal multiphoton microscopy Copyright Blackwell Science Ltd KEYWORDS Beam splitter • laser scanning microscopy • multifocal • two-photon ABSTRACTIn this article we present the development of a multibeam two-photon laser scanning microscope. A new type of beam splitter to create the multitude of laser beams is described. This type of beam splitter has higher transmission and generates more uniform beams than can be achieved with the microlens approach used by other groups. No crosstalk exists between the different foci due to small temporal delays between the individual beams. The importance of dispersion compensation to obtain maximum efficiency of the microscope is discussed. With optimum compensation the fluorescence signal was raised by a factor of 14. Different modes of detecting the fluorescence signals and their effect on imaging speed and resolution are discussed. Received 18 May 2000; accepted 15 November 2000 | 
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