Genetic disruption of the respiratory NADH-ubiquinone reductase of Rhodobacter capsulatus leads to an unexpected photosynthesis-negative phenotype

doi:10.1111/j.1574-6968.1997.tb10275.x

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Wiley InterScience

FEMS Microbiology Letters

Volume 148 Issue 1, Pages 107 - 114

Published Online: 17 Jan 2006

Published on behalf of the Federation of European Microbiological Societies

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Genetic disruption of the respiratory NADH-ubiquinone reductase of Rhodobacter capsulatus leads to an unexpected photosynthesis-negative phenotype

Alain Dupuis ^a *, André Peinnequin ^a ¹ , Elisabeth Darrouzet ^a , Joël Lunardi ^a

^a Laboratoire de Bioénergétique Cellulaire et Pathologique (BECP), Département de Biologie Moléculaire et Structurale, CEA Grenoble, 17 rue des martyrs, 38054 Grenoble Cédex, France

*Corresponding author. Tel.: +33 (4) 76 88 58 37; fax: +33 (4) 76 88 51 87; e-mail: dupuis@aup.ceng.cea.fr

¹ CRSSA, Av. Maquis du Grésivaudan, 38700 La Tronche, France.

KEYWORDS

NADH-ubiquinone oxidoreductase • Lactate dehydrogenase • Photosynthesis deficiency • Respiratory chain • Rhodobacter capsulatus

ABSTRACT

The NADH-ubiquinone reductase (type I NDH) of Rhodobacter capsulatus is a good model for the genetic study of bacterial homologues of the mitochondrially encoded ND subunits of the mitochondrial complex I. We disrupted the genes nuoH and nuoL which code for the bacterial homologues of subunits ND1 and ND5, respectively, of the mitochondrial enzyme. The corresponding NHK1 and NLK1 mutants present very low membrane-bound NADH-ubiquinone reductase activity. However, these mutants had an unexpected phenotype: while they displayed a nearly normal respiratory growth phenotype on lactate, they were unable to grow photosynthetically under anaerobiosis. Based on the observation of NADH-independent l(−) lactate dehydrogenase (iLDH) activity in membranes of R. capsulatus, we propose a metabolic explanation for this apparently aberrant phenotype.

Received 6 December 1996, Revised 13 January 1997, Accepted 13 January 1997

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1574-6968.1997.tb10275.x About DOI