Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas

doi:10.1046/j.1444-2906.2000.00013.x

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Wiley InterScience

Fisheries Science

Volume 66 Issue 1, Pages 91 - 96

Published Online: 25 Dec 2001

The Official Journal of the Japanese Society of Fisheries Science

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Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas

Qi Li, ¹ * Makoto Osada, ¹ Masaru Kashihara, ² Ken Hirohashi ² and Akihiro Kijima ¹

¹ Education and Research Center of Marine Bio-resources, Faculty of Agriculture, Tohoku University, Onagawa, Miyagi 986-2242 and ² Biomate Co. Ltd, Higashikasai, Edogawa, Tokyo 134-0084, Japan

KEYWORDS

gynogenesis • Pacific oyster • sperm morphology • ultraviolet irradiation

ABSTRACT

SUMMARY:

Effects of ultraviolet (UV) irradiation on genetical inactivation and morphological features of sperm were examined in the Pacific oyster. Ultraviolet light (254 nm) was effective for inactivating sperm chromosomes. Irradiation for 60 s at a UV intensity of 72 erg/mm ² per s was the optimum dose to achieve haploid gynogenesis. The rates of the fertilization and the development of D-shaped larvae decreased with increasing irradiation time, and the development of the eggs fertilized with the genetically inactivated sperms terminated before reaching the D-shaped stage. Electron microscopy showed clear destruction of the sperm acrosome and flagellum in the UV-irradiated sperms. As the duration of irradiation increased, the acrosome of sperms tended to suffer greater damage until the sperms eventually lost their flagella. Abnormalities in these structures have appeared to account, at least in part, for the decline of the fertilization rate of eggs inseminated with UV-irradiated sperms.

DIGITAL OBJECT IDENTIFIER (DOI)

10.1046/j.1444-2906.2000.00013.x About DOI