Addition of Colchicine to Wheat Anther Culture Media to Increase Doubled Haploid Plant Production

doi:10.1111/j.1439-0523.1994.tb00670.x

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Wiley InterScience

Plant Breeding

Volume 112 Issue 3, Pages 192 - 198

Published Online: 28 Apr 2006

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Addition of Colchicine to Wheat Anther Culture Media to Increase Doubled Haploid Plant Production

W. Navarro-Alvarez ^1, ² , P.S. Baenziger ^3, ⁶ , K.M. Eskridge ⁴ , M. Hugo ^5, ² V.D. Gustafson ³

¹ Escuela de Ciencias Agrarias, Universidad Nacional, Heredia, Costa Rica ² (formerly) Department of Agronomy, University of Nebraska, Lincoln, NE 68583-0915, USA ³ Department of Agronomy, University of Nebraska, Lincoln, NE 68583-0915, USA ⁴ Department of Biometry, University of Nebraska, Lincoln, NE 68583-0712, USA ⁵ Department of Genetics, University of Stellenbosch, Stellenbosch 7600, RSA

⁶ Corresponding author.

With 3 tables

Communicated by C. O. Qualset

KEYWORDS

Triticum aestivum • wheat starch • anther culture • doubled haploids • colchicine • sugars • genotype dependence

ABSTRACT

Chromosome doubling is critical for obtaining doubled-haploid plants from wheat (Triticum aestivum L.) anther culture. The most common doubling method applies colchicine to the plant. However, colchicine is phytotoxic and can induce a high frequency of plant death. In this experiment, anthers from two wheat genotypes ("Pavon 76" and 'Centurk') were placed on nine embryoid initiation media having three sugar sources (maltose, sucrose, and maltose + glucose) with three colchicine concentrations (0.0, 0.1, and 0.2 g · l^-1). Wheat starch was used as a gelling agent. After three days, the anthers were washed and moved to fresh media without colchicine. Increasing the colchicine concentration decreased the number of embryoids produced from 77.4 embryoids/100 anthers to 29.9 embryoids/100 anthers, but did not significantly affect the frequency of plant regeneration (0.49 green plants/embryoid to 0.40 green plants/embryoid), and increased the frequency of doubled-haploid plants (19.0 doubled-haploid plants/100 green plants to 72.3 doubled-haploid plants/100 green plants). Considering the total number of doubled-haploid plants produced, low levels of colchicine added to the initiation media were very effective.

Received February 12, 1993/Accepted September 17, 1993

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1439-0523.1994.tb00670.x About DOI