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Wiley InterScience

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 109 Issue 2-3, Pages 279 - 282

Published Online: 17 Jan 2006

© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved



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Transformation and expression of a staphylococcal plasmid in Escherichia coli
Dilip Saha 1 and Manjusri Bal 1
  1 Department of Physiology, Section of Immunology and Microbiology, University College of Science and Technology, Calcutta, India
 Correspondence to: D. Saha, Department of Physiology, Section of Immunology and Microbiology, University College of Science and Technology, 92, A.P.C. Road, Calcutta-700 009, India.
Copyright 1993 Federation of European Microbiological Societies
KEYWORDS
Plasmid • S. aureus • Transformation • Escherichia coli

ABSTRACT

Abstract A multiple antibiotic-resistant Staphylococcus aureus, was found to possess three plasmid bands in agarose gel electrophoresis. A plasmid of approximately 4.3 kb (pMC790/2) was found to code for ampicillin and tetracycline resistance and to have one EcoRI site when transformed into S. aureus RN 4220. pMC790/2 in unmodified form was transformed into a recAE. coli at a frequency of 1.2×104 transformants/μg of plasmid DNA. Plasmid (pMC790/2) replicated, maintained itself stably and expressed far better in the E. coli host than in S. aureus.


(Received 2 October 1992, Revised 7 January 1993, Accepted 21 January 1993)

DIGITAL OBJECT IDENTIFIER (DOI)
10.1111/j.1574-6968.1993.tb06181.x About DOI

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