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Wiley InterScience | ||||
  Molecular MicrobiologyVolume 5 Issue 5, Pages 1013 - 1022 Published Online: 27 Oct 2006 Journal compilation © 2010 Blackwell Publishing
Abstract | References | Full Text: PDF (Size: 3570K) | Related Articles | Citation Tracking  Molecular analysis of a complex locus from Haemophilus influenzae invoked in phase-variable lipopolysaccharide biosynthesis Copyright 1991 Blackwell Publishing ABSTRACT
A chromosomal locus, lic3, one of several involved in lipopolysaccharide (LPS) biosynthesis by Haemophilus influenzae, was cloned and its DNA sequence determined. Iic3 comprises four closely apposed open reading frames (ORFs). ORF1 includes tandem repeats of the tetramer CAAT and two start codons out of frame with each other are found upstream of the repeats. ORF1 encodes a protein with no known homologues. 0RF2 encodes the UDP-galactose-4-epimerase (galE) gene. ORF3 encodes a hydrophobic protein with no known homologues. 0RF4 encodes the adenylate kinase (adk) gene. A deletion/insertion mutation lacking the 3' end of ORF1, all of galE, and the 5' end of ORF3 was constructed in the parent Hib strain (RM7004). These mutants had a galE phenotype, as evidenced by galactose sensitivity, altered LPS when grown in the absence of exogenous galactose, and reduced virulence in infant rats. Received 12 December, 1990, revised 17 January, 1991. |
