Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development

doi:10.1111/j.1365-2443.2008.01164.x

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Wiley InterScience

Genes to Cells

Volume 13 Issue 3, Pages 221 - 231

Published Online: 21 Feb 2008

Published on behalf of the Molecular Biology Society of Japan

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Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development

Pazit Oren-Giladi ¹ , Ofra Krieger ² , Bruce A. Edgar ³ , Daniel A. Chamovitz ² and Daniel Segal ^1,*

¹ Department of Molecular Microbiology and Biotechnology, and ² Department Plant Sciences, Tel Aviv University, Tel Aviv, Israel ³ Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

* Correspondence: E-mail: dsegal@post.tau.ac.il

Communicated by: Xing-Wang Deng

ABSTRACT

The COP9 signalosome (CSN) is a multisubunit regulator highly conserved in evolution. We show here that CSN subunit 8 (CSN8) is essential for Drosophila development. CSN8 is maternally contributed and present throughout development. Null mutants generated in this study are larval lethal, showing phenotypes associated with mutations in either CSN4 (molting defects) or CSN5 (melanotic tumors). Analysis of mitotic and germ-line csn8^null clones revealed the requirement of CSN8 for multiple developmental processes. The germ-line clones arrested at mid-oogenesis, while the mitotic clones led to deformed adult eyes or wings. CSN8 is present exclusively as part of the CSN holo-complex, and lack of CSN8 in the mutants leads to CSN instability. Consistent with this, Cullin deneddylation is impaired in the csn8^null mutants.

Received: 30 August 2007 Accepted: 20 November 2007

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1365-2443.2008.01164.x About DOI