Soybean defense responses to the soybean aphid

doi:10.1111/j.1469-8137.2008.02443.x

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Wiley InterScience

New Phytologist

Volume 179 Issue 1, Pages 185 - 195

Published Online: 14 Apr 2008

Published on behalf of the New Phytologist Trust

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Soybean defense responses to the soybean aphid

Yan Li ¹ , Jijun Zou ² , Min Li ² , Damla D. Bilgin ³ , Lila O. Vodkin ² , Glen L. Hartman ^2,4 and Steven J. Clough ^2,3,4

¹ Physiological and Molecular Plant Biology Program, University of Illinois at Urbana-Champaign, IL, USA; ² Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL, USA; ³ Institute for Genomic Biology, University of Illinois at Urbana-Champaign, IL, USA; ⁴ USDA-ARS and National Soybean Research Center, Urbana, IL, USA

Author for correspondence: G. L. Hartman Tel: +1 217 244 3258 Fax: +1 217 244 7703 Email: ghartman@uiuc.edu

Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable.

KEYWORDS

cDNA microarray • incompatible interaction • plant–aphid interaction • plant defense • plant resistance • soybean aphid

New Phytologist (2008) 179: 185–195
No claim to original US government works. Journal compilation ©New Phytologist (2008)
doi: 10.1111/j.1469-8137.2008.02443.x

ABSTRACT

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Transcript profiles in aphid (Aphis glycines)-resistant (cv. Dowling) and -susceptible (cv. Williams 82) soybean (Glycine max) cultivars using soybean cDNA microarrays were investigated.
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Large-scale soybean cDNA microarrays representing approx. 18 000 genes or c. 30% of the soybean genome were compared at 6 and 12 h post-application of aphids. In a separate experiment utilizing clip cages, expression of three defense-related genes were examined at 6, 12, 24, 48, and 72 h in both cultivars by quantitative real-time PCR.
•
One hundred and forty genes showed specific responses for resistance; these included genes related to cell wall, defense, DNA/RNA, secondary metabolism, signaling and other processes. When an extended time period of sampling was investigated, earlier and greater induction of three defense-related genes was observed in the resistant cultivar; however, the induction declined after 24 or 48 h in the resistant cultivar but continued to increase in the susceptible cultivar after 24 h.
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Aphid-challenged resistant plants showed rapid differential gene expression patterns similar to the incompatible response induced by avirulent Pseudomonas syringae. Five genes were identified as differentially expressed between the two genotypes in the absence of aphids.