If you are seeing this message, you may be experiencing temporary network problems. Please wait a few minutes and refresh the page. If the problem persists, you may wish to report it to your local Network Manager.
It is also possible that your web browser is not configured or not able to display style sheets. In this case, although the visual presentation will be degraded, the site should continue to be functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise these problems.
Wiley InterScience | ||
 The Prostate JournalVolume 1 Issue 2, Pages 88 - 92 Published Online: 5 Jan 2002 Blackwell Science Inc
Abstract | References | Full Text: HTML, PDF (Size: 492K) | Related Articles | Citation Tracking  Prostate Apoptosis in Response to Castration in Wild-Type and Nerve Growth Factor-Induced Gene A-Deficient Mice Copyright 1999, Blackwell Science, Inc. ABSTRACTObjective: Nerve growth factor-induced gene A (NGFIA) is a transcription factor implicated in androgen deprivation-induced apoptosis in an androgen-sensitive prostate cell line ( 1, 2). The objective of our study was to investigate the role of NGFIA in prostate apoptosis in response to androgen ablation in a mouse animal model lacking the gene. Materials and Methods: Wild-type mice (n = 56) and NGFIA-deficient mice ("knock-out") (n = 16) were surgically castrated. The animals were killed at 0 (noncastrated controls), 1, 3, 5, 7, 10, 14, and 21 days after castration, and the prostates were harvested. Tissue sections were stained for morphologic analysis and quantification of apoptosis using a terminal deoxynucleotidyl transferase biotinylated deoxyuridine triphosphate nick-end labeling (TUNEL) strategy. Apoptosis was quantitatively measured by counting the number of TUNEL-positive cells/100 epithelial cells by light microscopy. The percentage of apoptosis was compared for wild-type mice versus NGFIA-deficient mice after castration at the defined time points. Results: We found a statistically significant increase in the mean percentage of prostate cell apoptosis within 7–21 days after castration in both wild-type and NGFIA-deficient mice (p < 0.05). However, there was no statistically significant difference in the mean percentage of apoptotic prostate cells between wild-type and NGFIA-deficient mice 1–5 or 7–21 days after castration (p > 0.05). Conclusion: NGFIA does not seem to play a critical role in prostate apoptosis induced by androgen ablation in this mouse model. |  |
