Characterization of polymorphic microsatellite loci for the invasive monk parakeet (Myiopsitta monachus)

doi:10.1111/j.1471-8286.2007.01749.x

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Wiley InterScience

Molecular Ecology Notes

Volume 7 Issue 6, Pages 990 - 992

Published Online: 15 Mar 2007

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PRIMER NOTE

Characterization of polymorphic microsatellite loci for the invasive monk parakeet (Myiopsitta monachus)

M. A. RUSSELLO*, V. SARANATHAN†, S. BUHRMAN-DEEVER‡, J. EBERHARD§ and A. CACCONE†¶

*Unit of Biology and Physical Geography, University of British Columbia Okanagan, 3333 University Way, Kelowna, British Columbia, Canada V1V 1V7, †Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520, USA, ‡Department of Neurobiology and Behaviour, Cornell University Ithaca, NY 14853, USA, §Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA, ¶Yale Institute for Biospheric Studies, Yale University, New Haven, CT 06520, USA

Correspondence: M. A. Russello, Fax: # 250-807-8005; E-mail: michael.russello@ubc.ca

KEYWORDS

dinucleotide • invasive species • microsatellites • polymorphic • primers • Psittacidae

ABSTRACT

Microsatellite loci were characterized for the monk parakeet (Myiopsitta monachus) from a GT_n-enriched genomic library. Twelve of 14 microsatellite loci were polymorphic, averaging 6.7 alleles per locus across the 20 individuals genotyped. Mean expected heterozygosity was 0.72, with locus-specific values ranging from 0.53 to 0.90. An equally high multilocus probability of identity (2.48 × 10⁻¹²) was revealed for this set of loci. In addition, all 12 loci were demonstrated to cross-amplify to varying extents within three additional parrot genera suggesting their potential utility for population-level studies in a broad range of Neotropical psittacines.

Received 14 January 2007; revision accepted 7 February 2007

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1471-8286.2007.01749.x About DOI