Polymorphic microsatellite DNA markers for the Florida manatee (Trichechus manatus latirostris)

doi:10.1111/j.1471-8286.2007.01781.x

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Wiley InterScience

Molecular Ecology Notes

Volume 7 Issue 6, Pages 1073 - 1076

Published Online: 10 Apr 2007

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PRIMER NOTE

Polymorphic microsatellite DNA markers for the Florida manatee (Trichechus manatus latirostris)

KIMBERLY C. PAUSE*, CORALIE NOURISSON†^,**, ANNMARIE CLARK‡, MARGARET E. KELLOGG§, ROBERT K. BONDE¶ and PETER M. McGUIRE*

*University of Florida, Department of Biochemistry and Molecular Biology, Box 100245 UFHSC, Gainesville, FL 32610, USA, †Center d'Oceanographie de Marseille, France, ‡University of Florida Interdisciplinary Center for Biotechnology Research, Genetic Analysis Laboratory, 1376 Mowry Road, Gainesville, FL 32610, USA, §University of Florida, College of Veterinary Medicine, Department of Physiological Sciences, Box 100245 UFHSC, Gainesville, FL 32610, USA, ¶Sirenia Project, Florida Integrated Science Center, US Geological Survey, 2201 NW 40th Terrace, Gainesville, FL 32605, USA

Correspondence: P. M. McGuire, Fax: (352) 392–2953; E-mail: pmcguire@biochem.med.ufl.edu

**Current address: ECOSUR, Proyecto Manati, Avenue Centenario km 5.5, CP 77900, Chetumal Q. Roo, Mexico,

KEYWORDS

DNA markers • Florida manatee • genetics • microsatellites • Sirenia

ABSTRACT

Florida manatees (Trichechus manatus latirostris) are marine mammals that inhabit the coastal waters and rivers of the southeastern USA, primarily Florida. Previous studies have shown that Florida manatees have low mitochondrial DNA variability, suggesting that nuclear DNA loci are necessary for discriminatory analyses. Here we report 10 polymorphic microsatellite loci with an average of 4.2 alleles per locus, and average heterozygosity of 50.1%. These loci have been developed for use in population studies, parentage assignment, and individual identification.

Received 6 January 2007; revision accepted 6 March 2007

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1471-8286.2007.01781.x About DOI