If you are seeing this message, you may be experiencing temporary network problems. Please wait a
few minutes and refresh the page. If the problem persists, you may wish to report it to your local
Network Manager.
It is also possible that your web browser is not configured or not able to display style sheets.
In this case, although the visual presentation will be degraded, the site should continue to be
functional. We recommend using the latest version of Microsoft or Mozilla web browser to help minimise
these problems.
Wiley InterScience | |
|
 research-articleTissue Engineering with Chondrogenically Differentiated Human Embryonic Stem Cells | | Eugene J. Koay 2 1, Gwen M. B. Hoben 2 1, Kyriacos A. Athanasiou, Ph.D., P.E. 1 * | 1Department of Bioengineering, Rice University, Houston, Texas, USA
2Baylor College of Medicine, Houston, Texas, USA
| | email: Kyriacos A. Athanasiou (athanasiou@rice.edu) |
*Correspondence to Kyriacos A. Athanasiou, Rice University, Department of Bioengineering, MS-142, P.O. Box 1892, Houston, Texas 77251-1892, USA. Telephone: (713) 348-6385; Fax: (713) 348-5877 | Human embryonic stem cells • Chondrogenesis • Cartilage • Tissue engineering |
This study describes the development and application of a novel strategy to tissue engineer musculoskeletal cartilages with human embryonic stem cells (hESCs). This work expands the presently limited understanding of how to chondrogenically differentiate hESCs through the use of chondrogenic medium alone (CM) or CM with two growth factor regimens: transforming growth factor (TGF)- 3 followed by TGF-1 plus insulin-like growth factor (IGF)-I or TGF-3 followed by bone morphogenic protein (BMP)-2. It also extends the use of the resulting chondrogenically differentiated cells for cartilage tissue engineering through a scaffoldless approach called self-assembly, which was conducted in two modes: with (a) embryoid bodies (EBs) or (b) a suspension of cells enzymatically dissociated from the EBs. Cells from two of the differentiation conditions (CM alone and TGF-3 followed by BMP-2) produced fibrocartilage-like constructs with high collagen I content, low collagen II content, relatively high total collagen content (up to 24% by dry weight), low sulfated glycosaminoglycan content ( 4% by dry weight), and tensile properties on the order of megapascals. In contrast, hESCs treated with TGF-3 followed by TGF-1 + IGF-I produced constructs with no collagen I. Results demonstrated significant differences among the differentiation conditions in terms of other biochemical and biomechanical properties of the self-assembled constructs, suggesting that distinct growth factor regimens differentially modulate the potential of the cells to produce cartilage. Furthermore, this work shows that self-assembly of cells obtained by enzymatic dissociation of EBs is superior to self-assembly of EBs. Overall, the results of this study raise the possibility of manipulating the characteristics of hESC-generated tissue toward specific musculoskeletal cartilage applications. | | Disclosure of potential conflicts of interest is found at the end of this article. |
Received: 7 February 2007; Accepted: 17 May 2007 10.1634/stemcells.2007-0105 About DOI
Related Articles- Find other articles like this in Wiley InterScience
- Find articles in Wiley InterScience written by any of the authors
Wiley InterScience is a member of CrossRef. 
| |
