Effects of primary testicular damage on sperm DNA oxidative status and embryonic and foetal development

doi:10.1111/j.1439-0272.2009.00929.x

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Wiley InterScience

Andrologia

Volume 41 Issue 5, Pages 282 - 296

Published Online: 7 Sep 2009

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ORIGINAL ARTICLE

Effects of primary testicular damage on sperm DNA oxidative status and embryonic and foetal development

F. Dimitriadis ¹ , D. Giannakis ² , N. Pardalidis ² , K. Tsoukanelis ² , N. Kanakas ² , M. Saito ¹ , T. Watanabe ¹ , I. Miyagawa ¹ , P. Tsounapi ¹ & N. Sofikitis ^1,2

1 Department of Urology, Tottori University School of Medicine, Yonago, Japan ;
2 Laboratory of Molecular Urology and Genetics of Human Reproduction, Department of Urology, Ioannina University School of Medicine, Ioannina, Greece

Correspondence to Nikolaos Sofikitis, Department of Urology, Tottori University School of Medicine, 36 Nischimachi, Yonago 683, Japan.
Tel.: +30 69 4436 3428;
Fax: +30 26 5109 7069;
E-mail: akrosnin@hotmail.com

Presented as a preliminary study at the 53rd Annual Meeting of The American Society for Reproductive Medicine in Cincinnati, Ohio, October 18th to 23rd, 1997.

KEYWORDS

Embryonic development • spermatozoa • varicocele • rat

ABSTRACT

We evaluated the development of embryos generated from the fertilisation of oocytes with spermatozoa isolated from animals with primary testicular damage (PTD). Embryos derived in vivo or in vitro from oocytes fertilised with spermatozoa produced by PTD rats that had undergone surgical treatment for the PTD (group A1), or PTD rats (group A2), or control rats (group B) were cultured and transferred to recipients. At the end of the experimental period, the fertilisation potential of each rat was assessed in vitro (IVF trials). Sperm 8-oxodG/dG ratio (a marker of DNA oxidative status) was significantly larger in group A2 than in groups A1 and B. Blastocysts of the group A2 transferred to recipients demonstrated a significantly larger loss before implantation than transferred blastocysts of groups A1 or B. In addition, the proportion of implanted blastocysts that could not complete the intrauterine development was significantly larger in group A2 than in groups A1 and B. This study reveals a post-fertilisation detrimental effect in animals with PTD on the capacity of oocytes (fertilised either in vitro or in vivo) to develop in vitro and implant after transferring them to recipients probably attributable to sperm DNA oxidative damage.

Accepted: February 4, 2009

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1439-0272.2009.00929.x About DOI