Ratio of myeloid and plasmacytoid dendritic cells and TH2 skew in CRS with nasal polyps

doi:10.1111/j.1398-9995.2009.02174.x

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Wiley InterScience

Allergy

Volume 65 Issue 1, Pages 24 - 31

Published Online: 30 Sep 2009

Published with the European Academy of Allergy and Clinical Immunology (EAACI)

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ORIGINAL ARTICLE

Ratio of myeloid and plasmacytoid dendritic cells and T_H2 skew in CRS with nasal polyps

H. Kirsche ¹ , A. Niederführ ¹ , T. Deutschle ¹ , C. Fuchs ¹ & H. Riechelmann ²

¹ Department of Otorhinolaryngology – Head and Neck Surgery, University Hospital of Ulm, Ulm, Germany ; ² Department of Otorhinolaryngology – Head and Neck Surgery, Medical University, Innsbruck, Austria

Correspondence to Herbert Riechelmann, Department of Otorhinolaryngology – Head and Neck Surgery, Medical University, Anichstraße 35, 6020 Innsbruck, Austria.

Edited by: Wytske Fokkens

KEYWORDS

cytokines • dendritic cells • Helper-Inducer • nasal polyps • sinusitis • Staphylococcus aureus • T-lymphocytes

To cite this article: Kirsche H, Niederführ A, Deutschle T, Fuchs C, Riechelmann H. Ratio of myeloid and plasmacytoid dendritic cells and T_H2 skew in CRS with nasal polyps. Allergy 2010; 65: 24–31

ABSTRACT

Background: The role of myeloid and plasmacytoid dendritic cells and its consequences for the T_H2 skew in chronic rhinosinusitis (CRS) with nasal polyps (CRSNP⁺) should be detailed.

Methods: In 18 CRS patients without nasal polyps (CRSNP⁻), 35 CRSNP⁺ patients and 22 patients with nasal structural abnormalities without rhinosinusitis (controls), dendritic cells (DC) were differentiated into myeloid (mDC) and plasmacytoid (pDC) subtypes using an antibody cocktail including CD1c (BDCA-1) and CD303 (BDCA-2) in peripheral blood mononuclear cells (PBMC) and single cell preparations of sinonasal mucosa by flow cytometry. Moreover, cells were analysed for expression of CD45, CD3, CD4, CXCR3 (T_H1) and CCR4 (T_H2) and IFN-γ, IL-5, TGF-β1, TGF-β2, ECP and total IgE in nasal secretions were determined. As a possible confounder, Staphylococcus aureus in nasal lavages was detected.

Results: The tissue mDC/pDC-ratio was 1.7 (1.0–2.4) in controls, 3.0 (1.8–4.0) in CRSNP⁻ and 0.8 (0.6–1.0) in CRSNP⁺ (P < 0.01). In tissue samples, the T_H1/T_H2 ratio was 12.6 (6.4–16.0) in controls, 12.5 (6.9–21.2) in CRSNP⁻ and 1.8 (1.3–3.6) in CRSNP⁺ (median and interquartile range, P < 0.001). Less pronounced differences were found in PBMC. S. aureus detection rates or TGF-β levels did not differ between patient groups and S. aureus detection had no influence on the parameters investigated.

Conclusion: A significant T_H2 skew in CRSNP⁺ could be confirmed on the cellular level. It was driven by low myeloid dendritic cell numbers. The T_H2 skew did not correlate with S. aureus detection. The data support the concept that CRSNP⁺ and CRSNP⁻ are pathophysiologically distinct.

Accepted for publication 30 June 2009

DIGITAL OBJECT IDENTIFIER (DOI)

10.1111/j.1398-9995.2009.02174.x About DOI